Skip to main content
. 2017 Nov 1;7(11):2209–2219.

Figure 2.

Figure 2

Down-regulation of S100A4 phenocopied the effect of miR-149-3p. (A and B) Expression of S100A4 in UM-UC-3 cells was measured by western blot (A) and Rt-PCR (B) after transfection with siRNA against S100A4 (n=5). Data are presented as the mean ± SD (***P < 0.001 versus control group). (C) The CCK-8 assay showed that the relative viabilities of UM-UC-3 cells were significantly decreased after down-regulating the expression of S100A4 (n=5). Data are presented as the mean ± SD (*P < 0.05, ***P < 0.001 versus control group). (D and E) Colony formation assay showed that the colony formation rates of UM-UC-3 cells were decreased after transfection with siS100A4 (n=5). Data are presented as the mean ± SD (***P < 0.001 versus NC group). (F) Wound healing assays indicated that suppression of S100A4 expression reverses the migration of UM-UC-3 cells. (G-I) Transwell assays confirmed that down-regulation of S100A4 expression inhibited the migration and invasion of UM-UC-3 cells (n=5). Data are presented as the mean ± SD (***P < 0.001 versus NC group).