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. 2017 Nov 1;7(11):2209–2219.

Figure 3.

Figure 3

Ectopic expression of S100A4 restores the phenotypes of BCa cell lines transfected with miR-149-3p. A. Western blot shows the expression of S100A4, E-cadherin, and vimentin in UM-UC-3 cells after ectopic expression of S100A4. B. The CCK-8 assay shows that S100A4 rescues the capacity of relative viabilities decreased in UM-UC-3 cells after up-regulation of miR-149-3p expression (n=5). Data are presented as the mean ± SD. *P < 0.05 (***P < 0.001 versus control group). C and D. Colony formation assay showed that the colony formation rates of UM-UC-3 cells recovered with up-regulation of S100A4 expression after transfection with miR-149-3p mimics (n=5). Data are presented as the mean ± SD (***P < 0.001 versus control group; ###P < 0.001 versus mimic group). E. Wound healing assays indicated that the expression of S100A4 reversed the inhibitory effect of miR-149-3p on UM-UC-3 cell migration. F-H. Transwell assays confirmed that up-regulation of S100A4 expression reverses the inhibitory effect of miR-149-3p on UM-UC-3 cell migration (F and G) and invasion (F and H).