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. 2017 Nov 1;7(11):2234–2244.

Figure 3.

Figure 3

AKIP1 promoted the migration, invasion and epithelial-mesenchymal transition of NSCLC cells. A, B. AKIP1 silencing inhibited the migratory and invasive ability of A549 cells. C, D. The effect of knockdown of AKIP1 on epithelial and mesenchymal markers was evaluated by immunoblotting and qRT-PCR analysis, respectively. E, F. Overexpression of AKIP1 promoted the migratory and invasive ability of NCI-H1299 cells. G, H. Immunoblotting and qRT-PCR analysis showed that overexpression of AKIP1 promoted the expression of mesenchymal markers, while inhibited the expression of epithelial marker E-cadherin. I, J. The effect of AKIP1 on several transcriptional repressors of E-cadherin was evaluated by qRT-PCR assay and confirmed by immunoblotting assay. All experiments were performed at least three times. **P < 0.01.