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. 2017 Nov 1;7(11):2234–2244.

Figure 4.

Figure 4

AKIP1 regulated ZEB1 promotor activity through a SP1 dependent manner. Transcriptional repression (A) and activation (B) of ZEB1 promotor by AKIP1, as indicated by dual luciferase reporter assay system. (C) Serially deletion analysis in A549 cells identified an AKIP1-responsive region within the ZEB1 promoter. (D, F) ChIP assay showed a physical association between AKIP1 and ZEB1 promotor. (E) The interaction between AKIP1 and SP1 was confirmed by IP assay. Left: Cell lysates of A549 cells were immunoprecipitated with anti-AKIP1 and immunoblotted with anti-SP1 (first line)/anti-AKIP (second line); right: Cell lysates of A549 cells were immunoprecipitated with anti-SP1 and immunoblotted with anti-AKIP1 (first line)/anti-SP1 (second line). (G) Knockdown of SP1 in A549 cells inhibited the binding efficiency of AKIP1 within ZEB1 promotor. **P < 0.01.