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. 2017 Nov 1;7(11):2234–2244.

Figure 5.

Figure 5

ZEB1 was responsible for AKIP1 induced effects. A. NCI-H1299/AKIP1 cells were transiently transfected with scrRNA or siAKIP1, and the knockdown efficiency of AKIP1 was confirmed by western blot assay. B. Depletion of ZEB1 in NCI-H1299/AKIP1 cells inhibited the cell proliferation by CCK8 assay. C. Depletion of ZEB1 in NCI-H1299/AKIP1 cells inhibited the colony formation assay. D. Depletion of ZEB1 in NCI-H1299/AKIP1 cells inhibited cell migration/invasion ability. E. A549/shAKIP1 cells were transiently transfected with ZEB1 expressing plasmid or empty vector, and ZEB1 restoration was examined by western blot assay. F-H. Forced expression of ZEB1 in A549/shAKIP1 cells restored the proliferation, colony formation, migration and invasion ability. *P < 0.05, **P < 0.01.