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. 2017 Nov 1;7(11):2257–2274.

Figure 5.

Figure 5

KLK4 and KLK14 immunoprecipitate with hepsin and TMPRSS2. Western blot analyses were performed on immunoprecipitates from lysates of COS-7 cells transiently expressing proteases of interest. A. Cells transiently expressed KLK4-V5 and wild type or active-site mutant hepsin-Flag. Anti-Flag, anti-V5 and control immunoglobulin (IgG) precipitates were examined by anti-V5 (left) and anti-Flag (right) Western blot analysis. Asterisk indicates 17 kDa hepsin-generated KLK4 fragment. B. Cells transiently expressed KLK4-V5 and wild type or active-site mutant TMPRSS2-Myc. Anti-Myc, anti-V5 and control IgG precipitates were examined by anti-V5 (left) and anti-Myc (right) Western blot analysis. Asterisk indicates 15-17 kDa TMPRSS2-generated KLK4 fragment. C. Cells transiently expressed KLK14-HA and wild type or active-site mutant hepsin-Flag. Anti- Flag, anti-HA and control IgG precipitates were examined by anti-HA (left) and anti-Flag (right) Western blot analysis. D. Cells transiently expressed KLK14-HA and wild type or active-site mutant TMPRSS2-Myc. Anti-Myc, anti-HA and control IgG precipitates were examined by anti-HA (left) and anti-Myc (right) Western blot analysis. Asterisk indicates 17 kDa TMPRSS2-generated KLK14 fragment. #, antibody heavy/light chain used for immunoprecipitation.