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. 2017 Nov 15;9(11):4785–4806.

Figure 7.

Figure 7

Expression of the differentiation marker (GFAP), stem cell markers (CD133, nestin and oct4) and HAS in response to HA in C6 cell lines. A. The expression of the gfap mRNA was detected using RT-PCR. B. GFAP protein expression in C6 cell lines was analyzed using flow cytometry. C. The expression of the cd133 mRNA was detected using RT-PCR. D. CD133 protein expression was analyzed in C6 cell lines using flow cytometry. E. The expression of the nestin mRNA was detected using RT-PCR. F. Western blot analyses of Nestin protein expression in C6 cell lines. G. The expression of the oct4 mRNA was detected in C6 cell lines using RT-PCR. H. Western blot analyses of Oct4 protein expression in C6 cell lines. I. The expression of the has1 mRNA was detected using RT-PCR. J. The expression of the has2 mRNA was detected using RT-PCR. K. Western blot analyses of HAS1 and HAS2 protein expression levels in C6 cell lines (*p value < 0.05).