Table 1.

List and sequences of primers used in the RT-PCR analysis

Annotation	Primer sequence	Cycle	T (°C)
Rat cd44	5’-TCCCACTATGACACATATTGC-3’	32	55
	5’-ACACCTTCTCCTACTGTTGAC-3’
Rat gfap	5’-CAAGCCAGACCTCACAGCG-3’	32	55
	5’-GGTGTCCAGGCTGGTTTCTC-3’
Rat cd133	5’-AAGCAGCAAGTTGCCGGAGGAA-3’	35	61
	5’-GTCATCTTCTCTGTGATGGCGTACA-3’
Rat nestin	5’-GCTACATACAGGACTCTGCTG-3’	35	58
	5’-AAACTCTAGACTCACTGGATTCT-3’
Rat oct4	5’-GAGGGATGGCATACTGTGGAC-3’	32	60
	5’-GGTGTACCCCAAGGTGATCC-3’
Rat has1	5’-GCTCTATGGGGCGTTCCTC-3’	35	57
	5’-CACACATAAGTGGCAGGGTCC-3’
Rat has2	5’-TCGGAACCACACTGTTTGGAGTG-3’	35	62
	5’CCAGATGTAAGTGACTGATTTGTCCC-3’
Rat β-actin	5’-CCTCTGGTCGTACCAC-3’	25	47.2
	5’-CAGTAACAGTCCGCCT-3’
Human cd44	5’-CCAACTCCATCTGTGCAG-3’	32	50.3
	5’-AACCTCCTGAAGTGCTGC-3’
Human gfap	5’-CGCGGCACGAACGAGTCC-3’	32	57
	5’-GTGTCCAGGCTGGTTTCTCG-3’
Human cd133	5’-CACTTACGGCACTCTTCACCT-3’	35	60
	5’-TGCACGATGCCACTTTCTCAC-3’
Human nestin	5’-GGGACAGAGTTCTCCGAGCT-3’	35	67
	5’-GAAGCCAGGACAGCAGGATC-3’
Human oct4	5‘-GTGGAGGAAGCTGACAACAA-3’	35	60
	5‘-GCCGGTTACAGAACCACACT-3’
Human nanog	5‘-CCTATGCCTGTGATTTGTGG-3’	35	57
	5’-GTTGTTTGCCTTTGGGACT-3’
Human β-actin	5’-TGGAATCCTGTGGCATCCATGAAAC-3’	25	57.7
	5’-TAAAACGCAGCTCAGTAACAGTCCG-3’