Table 2.
Cell cycle analysis of C6 cell lines. The cells were collected at the same density (approximately 3×106 cells/10 cm culture dish) and then analyzed according to PI fluorescence intensity using flow cytometry. The table shows the percentage of cells in the sub-G1, -G0/G1, -G2/M or -S phases of the cell cycle across the experimental C6 cell lines
| Cell Population | % of Gated Cells (mean ± SD) | |||
|---|---|---|---|---|
|
|
||||
| C6-WT | C6-mock | C6-sh1 | p value | |
| sub G1 | 1.74 ± 0.8 | 2.27 ± 2.28 | 2.34 ± 1.32 | 0.207315 |
| G0/G1 | 36.54 ± 1.59 | 39.88 ± 4.64 | 72.03 ± 1.16 | 0.00591¶ |
| S | 39.05 ± 2.39 | 36.52 ± 2.8 | 15.75 ± 0.72 | 0.002937¶ |
| G2/M | 22.65 ± 1.36 | 21.32 ± 4.4 | 9.87 ± 0.7 | 0.004046¶ |
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The significance of the difference between C6-WT and C6-sh1 cells.