Figure 6.

The number of CD8⁺ T cells undergone apoptosis was significant increased by using actin stabilization drug jasplakinolide. A: CD8⁺ T cells were treated with DMSO (0.05%) or jasplakinolide (1 μg/mL). Cells were harvested after 48 h incubation to analyze apoptosis rate using Annexin V/PI assay by flow cytometry. B: Histogram showing apoptosis rate of CD8⁺ T cells in JASP group was significant higher than the control group. C: Expression levels of β-actin protein in control group and JASP group was detected by western blot. All data representative of three independent experiments and calculated data are shown as mean ± SD. All statistical analyses were performed by student t test, ***p<0.001 compared to homogenic group.