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. 2017 Nov 15;9(11):4963–4973.

Figure 2.

Figure 2

miR-351 negatively regulated MSC osteogenic differentiation. The miR control or miR-351 or anti miR-351 were transfected into MSCs. The mRNA and protein levels of osteogenesis markers (ALP, collagen II) proteins were detected by qRT-PCR and western blot, respectively. A. AR-S indicated the level of calcium mineral deposition. B. qRT-PCR detection of mRNA expression of osteogenic markers in miR-351 or anti miR-351 transfected MSCs during osteogenic differentiation. C. Western blot detection of protein expression of osteogenic markers in miR-351 or anti miR-351 transfected MSC during osteogenic differentiation. D. Comparison of the density between the experimental groups. miR control group: MSCs transfected with miR control; miR-351 group: MSCs transfected with miR-351 mimic; anti miR-351 group: MSCs transfected with anti miR-351. *P<0.05 vs. miR control group. #P<0.05 vs. miR-351 group.