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. 2017 Nov 15;9(11):4963–4973.

Figure 3.

Figure 3

miR-351 directly targets VDR. To confirm whether miR-351 targeted the 3’-UTR of VDR, a luciferase activity assay was performed. MSCs were co-transfected with wild type VDR 3’-UTR (VDR-3’-UTR) or mutant VDR 3’-UTR (VDR-3’-UTR-mut) and miR-351 or anti miR-351. The relative luciferase activity was detected. A. The predicted miR-351 target sequence in the 3’UTR of VDR. B. Western blot detection of VDR protein expression in miR-351 or anti miR-351 transfected MSCs during osteogenic differentiation. C. Dual-luciferase reporter assay of MSCs transfected with the VDR-3’UTR reporter and miR-351 or anti miR-351. *P<0.05 vs. miR control. #P<0.05 vs. miR-351 group. D. Correlation analysis of miR-351 and VDR expression.