Figure 1.

Knockdown of HOTAIR suppressed glioma angiogenesis in vitro. A. HOTAIR was knocked down in A172 cells and real time RT-PCR analysis was performed to detect HOTAIR expression. B. Cell proliferation was measured using the MTT assay and the doubling time was calculated using the Patterson formula. HBMVECs treated for 24 h with the supernatant of A172 siHOTAIR transfectants showed significantly increased doubling time compared to that of the siRNA negative control supernatant. C. HBMVECs treated with A172 siHOTAIR supernatant formed significantly fewer colonies than the cells treated with the siRNA negative control supernatant. D. Migration of HBMVECs was measured using the transwell migration assay (magnification, 200×). The migration ability of HBMVECs was significantly inhibited after incubation with the A172 siHOTAIR supernatant. E. Tube formation was measured by the HBMVEC capillary tube formation assay, and the results were expressed as the number of branches (magnification, 100×). Data represent mean ± SD (N = 3, each). Con: control; si-NC: siRNA negative control; CM: conditional medium; *P<0.05, **P<0.001.