Skip to main content
. 2017 Nov 15;9(11):5022–5030.

Figure 4.

Figure 4

Downregulation of TLR2 attenuates the effect of PGA-sa on RANKL-induced osteoclastogenesis and activation of the NF-κB/NFATc1 signaling pathway. The inhibitory effect of si-TLR2 on TLR2 mRNA and protein expression was validated by qRT-PCR (A) and western blot analysis (B), respectively. (C) The promotoryeffect of PGA-sa on RANKL-induced osteoclastogenesis was attenuated by TLR2 knockdown. (D-G) qRT-PCR assay showed that downregulation of TLR2 by si-TLR2 reversed the upregulatory effect of PGA-saon mRNA expression of TRAP,cathepsin K, MMP9, and calcitonin receptor. (H) The representative image of western blot analysis of TLR2, p-IκBα, and NFATc1 protein expression. (I) Luciferase reporter gene assay showed that si-TLR2 attenuated the promotion of RANKLstimulated activation of NF-κB caused by PGA-sa. *P<0.05, **P<0.01, and ***P<0.001.