Table 2.

Preparation of PCR amplification reaction system

Reagent name	Usage amount (μl)
SYBR Premix Ex Taq (Tli RNaseH Plus) (2×)	10
PCR Forward Primer (10 μM)	0.8
PCR Reverse Primer (10 μM)	0.8
ROX Reference Dye II (50×)	0.4
cDNA solution	2
dH2O (Sterilized distilled water)	6
Total	20