Fig. 2.

a, b. Blastocysts undergo rapid ~ 30–50% decrease in Oct4 in and AMPK-dependent (CC-sensitive) manner. Morulae were cultured overnight to acclimate to culture to blastocyst stage, preloaded with CC (5 μM) for 2 h and then continued +/− CC with stimuli BR-DIM (20 μM) or metformin (40 μM), for an additional 1 h and then fixed, stained for Oct4 and Hoechst, micrographed quantitated, and graphed. Triplicate biological experiments were performed using 190 morulae, tested for normal distribution by ANOVA and significance by Tukey’s post hoc test. Significance compared with KSOM (a) and lack of significance for each stimulus with CC compared with the stimulus alone (b). White bar in first panel shows 25 μm