Fig. 3.

Two-cell stage embryos have AMPK agonist-induced (AICAR) and sorbitol-induced AMPK-dependent (compound C-sensitive) Oct4 losses. Compared with embryo cultured in KSOMaa (a, b), nuclear Oct4 is lost after 1 h of 200 mM (c, d) sorbitol but loss if largely reversed in the presence of 5 μM CC (e, f). AMPK agonist AICAR (0.5 mM) for 0.5 h (g, h) is sufficient to cause Oct4 loss without added stress). Eighty-three embryos were tested in triplicate experiments