Fig. 5.

Site-directed mutagenesis of the mouse wild-type Nt5e promoter-luciferase (F5) construct (−162 to −3 bp relative to mouse Nt5e gene start codon) was performed before transient expression in mouse NIH3T3 fibroblasts. The luciferase activity of a F5-Luc construct which contains a mutated mouse Elf2 transcription factor binding site was significantly higher than the one measured for the un-mutated F5-Luc construct, indicating that Elf2-like transcription factor acts as a potential repressor of mouse Nt5e gene expression (data are representative of, at least, three independent experiments)