Fig. 7.

a Relative mRNA expression of Nt5e and Elf2 genes is determined by quantitative real-time PCR analysis in quiescent and activated primary mouse HSC; mouse Gapdh was used as reference gene. b Expression of Nt5e and Elf2 mRNAs is determined by semi-quantitative PCR analysis in immortalized activated JS1 and Col-GFP mouse HSC, total mouse liver, and water (reaction control); mouse Gapdh was used as reference gene. c Electro-motility shift assays were performed using nuclear extracts obtained from mouse JS1 and Col-GFP cells, and digoxigenin-labeled probes corresponding to the minimal mouse Nt5e promoter containing the wild-type (Nt5e ^{wt Elf2} probe, left panel) and mutated (Nt5e ^{mut Elf2} probe, right panel) Elf2 motif (nucleotides −132 to −102) (see Table 1 for sequences). Competitive analysis was performed in the presence of a 125-fold molar excess of unlabeled competitors, as indicated. Arrowheads indicate formation of DNA-protein complexes (depicted image is representative of three independent experiments)