Table 1.
Specific primers for target and reference genes
| Gene symbol (transcript ID) | Forward | Reverse |
|---|---|---|
| rps18 (ENSDART00000017975) | ACCCTCGCCAGTACAAAATCC | CCTGATCTTCTTCAGCCTCTCC |
| β-Actin (ENSDART00000054987) | TCCCAAAGCCAACAGAGAGAAG | GTCACACCATCACCAGAGTCC |
| efl1-alpha (ENSDARG00000020850) | CAGCAGCTGAGGAGTGATCT | GTAGATCAGATGGCCGGTGG |
| ntpdase1 (ENSDART00000066257) | TCACAGACACAGCGAAACCC | GAATGTGCAACGGAGGTAAGC |
| ntpdase2-mq (ENSDART00000043259) | GAGCAGCAGCACGTAGCC | GACCTCAGCCGACTCTTTGG |
| ntpdase2-mv (ENSDART00000051434) | TGATGGAGTCTTTCAGCCCAAC | AGCAGCGTCCTCTAAATGAGC |
| ntpdase3 (ENSDART00000051167) | GGCTGGATCACTGTCAACTACC | CCCAAATCCAATGACCCAACTG |
| p2ry1 (ENSDART00000109100) | TATGGACAATGCTCCGCTTAGG | CGCTCTCATGTTCAGGTTCTTC |
| p2ry2 (ENSDART00000092965) | TCTGGTGTTTGTGTTCGGTCTG | ATGTAGATGGTGTTGGGCTTCC |
| p2ry11 (ENSDART00000008240) | CTTCCTCTTCACCAGCAACCTC | TGAACCACACCAGCACACTTG |
| p2ry12 (ENSDART00000102224) | AGCGTCTCCAACAGTTCATCC | GCCAGAGCGTTCAGGGATAATC |
| p2ry13 (ENSDART00000102221) | ACAAAGCAGCAGTGACGTAACC | GCGTCTGGCTGAGGGTGTAC |
| p2rx1 (ENSDART00000002866) | GGCAGGTGTATGAACAAAACCC | TCTGCTGTCAGTAGGATTGGAG |
| p2rx2 (ENSDART00000019461) | GCCAGAGAGAAATTCAGCGAAC | TATGTGGGAATACACGCAGACG |
| p2rx3a (ENSDART00000021417) | ATTCGCTTCCCTCTCTTTGGTG | AAAGATGGGACAGAACGGATGG |
| p2rx7 (ENSDART00000062229) | ACACCGAGAGGAAGTTTGAGG | AGGGGTTTTGTCTCCTGTAGTC |
| lin28a (ENSDART00000098970) | TTTCTGTCCATGACCCACCG | TCAGACTGCGAAAACCCTCC |
| ascl1a (ENSDART00000056005) | ACGACCCTCTGAGTCCAGAA | GACATCCTCCCAAGCGAGTG |
Primers were selected by using exonic sequences from the reported zebrafish genome (Ensembl database). RNA transcript identification number (ID) from the Ensembl database is shown in the first column. Selected primer pairs did not hybridize (at least five mismatches) with other sequences in the zebrafish genome (tested with primer BLAST)