Fig. 7.

Preparations of colonic longitudinal smooth muscle isolated from mice fed with normocaloric diet (NCD) or high-fat diet (HFD). a Effects of BAY 60-6583 (0.001–100 μM) on contractions evoked by rES (0.5 ms, 10 Hz, 30 mA, 10 s) in colonic preparations maintained in Krebs solution containing guanethidine, L-NAME, atropine (1 μM), GR159897 (NK₂ receptor antagonist 1 μM) SB218795 (NK₃ receptor antagonist, 1 μM) and added with dipyridamole (adenosine transport inhibitor, 0.5 μM) plus adenosine deaminase (the enzyme responsible for adenosine catabolism, 0.5 U/mL). b Column graphs showing the effects of BAY 60-6583 (1 μM), alone or in combination with MRS1754 (0.01 μM), on contractions elicited by sES (0.5 ms, 10 Hz, 30 mA, 10 s) in colonic preparations maintained in Krebs solution containing guanethidine, L-NAME, atropine (1 μM), and GR159897 (NK₂ receptor antagonist 1 μM) SB218795 (NK₃ receptor antagonist, 1 μM) and added with dipyridamole (adenosine transport inhibitor, 0.5 μM) plus adenosine deaminase (the enzyme responsible for adenosine catabolism, 0.5 U/mL). Each column represents the mean ± SEM obtained from six experiments. *P ˂ 0.05; ^a P ˂ 0.05 versus NCD plus BAY