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a Bone nodule formation in osteoblast cultures stained with Alizarin Red (AR-S). b Production of fibrillary collagen in osteoblast cultures measured by Sirius Red stain. c Alkaline phosphatase (ALP) activity measured as percentage of total protein content in cell lysates. AR-S data are presented as individual experiments (mean of four replicates), mean (—) and p value from Mann-Whitney U test (n = 4). Collagen and ALP data are presented as mean ± SEM and p value from t test (n = 10)
