Fig. 5.

Effects of nano emodin on α,β-meATP-activated current in HEK293 cells transfected with the hP2X3 receptor. a. α,β-meATP (5 μM) activated the currents in HEK293 cells transfected with the hP2X3 receptor. Nanocarrier has no inhibition effect to α,β-meATP-activated current. Normal emodin (30 μM) decreased α,β-meATP-activated current by 50%. b. Original tracings indicate that nano emodin (1.85 μM) primarily decreased α,β-meATP (5 μM)-induced currents by 60%. The histogram shows current density with different drugs. Data are presented as the means ± SEM of eight cells per group. *p < 0.05 compared with α,β-meATP alone. c. The photo shows the concentration-response curves for nano emodin against α,β-meATP (5 μM) with the IC₅₀ 1.87 ± 0.47