Fig. 4.

P2X7R and PanX1 antagonism after stretch injury reduces stretch-mediated extracellular ATP release in a subfailure overstretch model. Isolated rat abdominal aorta (RA) were either left untreated (NS) or stretched to twice the resting length. Stretched segments were then cut into 1–2 mm segments and incubated in heparinized PlasmaLyte for 10 min in the absence or presence of inhibitors to P2X7R/PanX1 complex: a BB FCF (50 μM), oATP (100 μM), or A740003 (100 μM) and b the pannexin inhibitory peptide ¹⁰PanX1 (200 μM), or carbenoxolone (CBX; 100 μM). ATP released were collected and concentration in the perfusates were measured (n = 6–25)