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. 2017 Nov 30;8:1629. doi: 10.3389/fimmu.2017.01629

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Copyright © 2017 Mulder, Banete, Seaver and Basta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Presentation of viral epitopes to CD8 T cells by LCMV-infected MΦ. (A) Polarized Sp-MΦ were infected with LCMV-WE (MOI 5) before testing for expression of LCMV-NP 24 h later. (B) In parallel, the same infected Sp-MΦ or uninfected negative controls were tested for their ability to directly present the LCMV GP33-41 or NP396-404 epitopes to their specific CTL cultures by assaying for IFN-γ production after in vitro stimulation by ICS. (C) Graphical representation of dot plots from B showing mean ± SD from three repeats. CTL, cytotoxic T lymphocyte; LCMV, lymphocytic choriomeningitis virus infection.