Figure 6.

The DNA-binding domain of TCP14 and TCP15 is required but not sufficient for the interaction with SCE1 in the Y2H assay. (A) Schematic representation of TCP14 and TCP15 fragments used in the Y2H assay. The numbers denote the residue positions in the full-length protein. Gray boxes represent the DNA-binding domain of the TCPs, while the white boxes depict putative SIMs. The predicted SUMO acceptor sites are indicated by K and the residue position. (B) Y2H protein interaction test between BD-SCE1 and fragments of TCP14 and TCP15. Yeast growth was scored after 3 days at 30°C. (C) Immunoblot showing the expression of the AD-TCP14/15 fragments in yeast. The fragments were detected using an antibody against the GAL4 AD. The molecular weights (kDa) for the protein standards are indicated on the left. The membranes were stained with Ponceau S to confirm equal loading of the protein extracts.