Table 1.

Summary of RNA-seq data.

Line	Temp °C	Replicate	PE reads	Median read quality R1	Median read quality R2	Trimmed PE reads	% mapped	% concordant	Estimated insert mean (bp)	Total observed genes	Mean expressed genes	% expressed genes
RBC2	33	A	17836372	37.1	36.9	16326998	89.9	82.3	216	16544	15351	73.1
		B	18039271	37.1	37.0	16541921	89.3	82.5	218	16622
	38	A	16252079	37.2	36.9	14822122	89.1	82.8	213	16365	15193	72.3
		B	16698643	37.1	37.0	15361039	89.2	83.3	198	16532
	43	A	15602450	37.2	37.0	14274919	89.7	83.4	226	16244	15399	73.3
		B	15303273	37.1	36.9	13986262	89.3	82.5	227	16397
F-Line	33	A	16169884	37.2	37.0	14865209	88.9	82.2	215	16557	15314	72.9
		B	17084981	37.2	37.0	15702722	89.5	82.8	215	16355
	38	A	18370285	37.2	37.0	16846899	89.9	83.5	217	16630	15270	72.7
		B	18085832	37.1	36.8	16419915	90.2	84.1	213	16575
	43	A	14634877	37.2	36.9	13373887	89.2	83.0	226	16498	15364	73.1
		B	15356625	37.2	36.9	14035364	89.2	82.9	226	16583
Mean			16619547.7	37.15	36.94	15213104.7	89.44	82.94	213.4	16491.8	15315.2	72.9

For each library the total number of concatenated reads, median read qualities (R1 and R2), estimated mean insert length (bp), number of and percentage of aligned reads, percentage of concordant reads, and the number and percentage of observed genes (mapped reads > 1.0) and expressed genes (mean group normalized read count > 3.0) are given.