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. 2017 Dec 4;7:16898. doi: 10.1038/s41598-017-16318-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Voxel positioning. An end systolic short-axis view (a) and a quasi-4-chamber view (b) of a volunteer’s heart are shown. The spectroscopy voxel was placed in the interventricular septum, with the effective acquisition volume indicated in orange. The red and white boxes indicate the excitation volume at 4.6 ppm (water frequency) and at 2.0 ppm, respectively, which differ due to chemical shift displacement. Inner-volume saturation (IVS) bands (blue) are used to saturate areas of the voxel that are subject to only partial excitation in the desired frequency range, due to chemical shift displacement, thus increasing localisation robustness.