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. 2017 Dec 4;7:16867. doi: 10.1038/s41598-017-17192-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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O₂ ⁻ increases KIF1Bβ protein expression in a positive feedback loop. (A) Immunoblot analysis of CHP212 cells transfected with 4 µg empty vector pcDNA3 (Empty) or FLAG-KIF1Bβ600–1400 for 24 hours followed by increasing doses of DPI treatment for 12 hours. (B) Immunoblot analysis of NB1 cells transfected with 4 µg empty vector pcDNA3 (Empty) or FLAG-KIF1Bβ600–1400 for 24 hours followed by increasing doses of DPI treatment for 12 hours. Bottom – corresponding densitometry for FLAG-KIF1Bβ600–1400 expression (mean ± SD; n = 3; *P < 0.5; ***P < 0.001). (C) Immunoblot analysis of KIF1Bβ expression in SK-N-SH cells in response to increasing doses of DDC treatment for 4 hours. Bottom – corresponding densitometry for KIF1Bβ expression (mean ± SD; n = 3). (D) Fold change in proteasomal activity with increasing doses of DDC treatment.