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. 2017 Dec 4;7:16883. doi: 10.1038/s41598-017-17113-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of AlstR activation on the firing frequency of post-inspiratory-modulated RVLM pre-sympathetic neuron recorded in situ. (A) Raw record of PN activity and whole cell current clamp recording of a post-inspiratory-modulated RVLM pre-sympathetic neuron from one in situ preparation of rat. Note that perfusion of Alst did not affect either the firing frequency or the post-inspiratory modulation of this pre-sympathetic neuron. (B) Whole cell current clamp recording of the same post-inspiratory-modulated pre-sympathetic neuron under blockade of fast synaptic transmission. Perfusion of Alst did not affect either the intrinsic pacemaker firing frequency or resting membrane potential. (C) The same post-inspiratory-modulated pre-sympathetic neuron labeled in situ with biocytin, depicting absence of either GFP or TH (a non-C1 neuron) immunofluorescence. Scale bar: 20 um.