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. 2017 Dec 4;7:16883. doi: 10.1038/s41598-017-17113-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of acute inhibition of C1 neurons on the respiratory and sympathetic activities of in situ preparations of control rats. (A) Raw and integrated (∫) records of tSN, AbN and PN activities from one in situ preparation of control rat transduced with PRSx8-AlstR-GFP-LVV into C1 region before and after arterial perfusion of Alst. PN-triggered averages of tSN and AbN from the same in situ preparation before (Bi) and after (Bii) Alst perfusion. Note that C1 neuronal inhibition reduced the amplitude of the tSN activity during I and PI, but affected neither the inspiratory (PN frequency and amplitude) nor expiratory (AbN) activities. Summary of data showing the changes in the PP (C), HR (D), baseline tSN activity (E) and in tSN activity during I, PI and E2 (F) 5 minutes after perfusion of Alst in in situ preparations of control rats transduced with PRSx8-AlstR-GFP-LVV into the C1 region.