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. 2017 Dec 4;7:16883. doi: 10.1038/s41598-017-17113-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of acute inhibition of C1 neurons on the autonomic, cardiovascular and respiratory responses to peripheral chemoreflex activation of in situ preparations of control rats. Raw and integrated (∫) recordings of PN, AbN, tSN activities, and PP and HR of one in situ preparation of a control rat, transduced with PRSx8-AlstR-GFP-LVV into the C1 region, illustrating the respiratory, sympathetic, pressure and bradycardic responses elicited by the activation of peripheral chemoreceptors with intra-arterial injection of KCN (0.05%, 50 μl) before (A) and after (B) arterial perfusion of Alst. (C) The percentage of average magnitude of the tSN reflex responses during I, expiration (E) and AbN (D) responses to peripheral chemoreflex activation in in situ preparations of control rats transduced with PRSx8-AlstR-GFP-LVV into the C1 region.