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. 2017 Dec 4;7:16887. doi: 10.1038/s41598-017-17177-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the three-dimensional (3D) organotypic matrix platform that demonstrates the two stages of the assay and concentration measurements from acid-extraction of collagen from rat and kangaroo tail tendons. (a) Stage 1 of the organotypic assay involves contraction of the rat or kangaroo tail collagen by fibroblasts, facilitating stromal-ECM assessment. (b) Stage 2 provides opportunities to assess cell invasion, proliferation and survival within a 3D co-culture environment. Application of (c) the Sircol^TM soluble collagen assay and (d) a modified Lowry assay to measure the collagen and total protein concentration of acid-extracted rat and kangaroo tail collagen respectively (n = 7), compared to commercial rat tail collagen (Corning, n = 3). (e) Values of collagen and total protein concentration from (c,d) were used to calculate the collagen abundance in each solution. Mean ± SD.