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. 2017 Dec 4;7:16873. doi: 10.1038/s41598-017-16802-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Oligomeric status of CXCR4 on the cell surface treated and untreated by CPZ. The experiments were carried out using the stably transfected T-Rex-HEK293 cells under resting state (without SDF-1α stimulation) and cells at different induction times were characterized by single-molecule imaging respectively. (A) Relative amount of monomer, dimer and oligomer of CXCR4-EGFP on the cell surface at induction times of 1, 2, 4, and 8 h without CPZ treatment; (B) Relative amount of monomer, dimer and oligomer of CXCR4-EGFP on the cell surface at induction times of 1, 2, 4, and 8 h with CPZ treatment.