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. 2017 Dec 4;8:1914. doi: 10.1038/s41467-017-02081-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commonslicense, unless indicated otherwise in a credit line to the material. If material is not included in the article’sCreative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — STIV virus infection induces TFS4 expression levels and growth retardation. a Infection time course experiment. The growth curve shows the increase of the optical density of S. solfataricus (strain 2-2-12) following infection by the STIV virus (t = 0). b, c Multiplex Western blot using polyclonal antibodies raised against TFS4 (b), RNAP subunits Rpo4/7 (c) and Alba (loading control) as a function of time following infection by STIV. Immunodetection was performed on two biological replicates. d Growth curves of S. acidocaldarius MW001 harbouring expression vectors encoding TFS1, TFS1-tip4, TFS1-tip4-AAA and LacS (control) under non-inducing or inducing e conditions using sucrose or maltose containing media, respectively. Error bars represent standard deviation from three technical repeats