Table 1.

Parameters of endothelin 1 and chymotrypsin captured with WtFraC and ReFraC

	WtFraC					ReFraC
	pH 7.5		pH 4.5			pH 7.5		pH 4.5
	τ on (ms)	τ off (ms)	τ on (ms)	τ off (ms)		τ on (ms)	τ off (ms)	τ on (ms)	τ off (ms)
Endothelin 1 (−50 mV)	–	–	5.8 ± 0.7	5.6 ± 2.0	Endothelin 1 (+50 mV)	1413.7 ± 286.9	3.3 ± 2.2	401.7 ± 79.4	8.5 ± 1.8
Chymotrypsin (−100 mV)	4.4 ± 1.9	12.0 ± 5.7	9.6 ± 2.5	1.7 ± 0.9	Chymotrypsin (+200 mV)	174.3 ± 22.9	0.2 ± 0.1	112.5 ± 9.5	1.3 ± 0.7

The buffer at pH 7.5 consisted of 1 M KCl, 15 mM Tris base. The buffer at pH 4.5 consisted of 1 M KCl, 0.1 M citric acid, 180 mM Tris base. Endothelin 1 and chymotrypsin were added into cis compartment. Recordings were performed using a 50 kHz sampling rate with a 10 kHz low-pass Bessel filter. The errors represent the standard deviations calculated from three experiments

τ _on inter-event time, τ _off dwell time