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. 2017 Dec 4;7:16863. doi: 10.1038/s41598-017-17319-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of two cell subsets in NB cell lines. (A) Flow cytometry analysis was performed to detect GFP expression in NB cells stably infected with the lentiviral SRR2 reporter. In both cell lines, a small subset of reporter responsive (RR) cells was found, whereas the majority of the cells were unresponsive to the reporter (i.e. reporter unresponsive, RU cells). NB cells stably infected with the mCMV (negative control) lentivirus were used as the negative control. (B) GFP expression in purified RU and RR cells purified from cells stably infected with the lentiviral SRR2 reporter were substantially different. (C) Cell growth of RU and RR cells were assessed by using the MTS assay. All data are presented as mean ± SD, *p < 0.05, Student’s t test. Abbreviations: NB, neuroblastoma; SRR2, Sox2 regulatory region 2; mCMV: Murine Cytomegalovirus; GFP: Green Fluorescence Protein.