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. 2017 Dec 4;7:16878. doi: 10.1038/s41598-017-17204-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Survival analysis of colon cancer cohort based on QuPath automated image analysis. (a–d) Kaplan Meier survival analysis for biomarker scores of TMAs stained for CD3, CD8, p53 and PD-L1. Median cutoffs are applied in all cases, except p53 where two cutoffs were selected by an experienced pathologist to distinguish between aberrant negative, “wild type” (normal) and aberrant positive groups. Representative images showing an original core and QuPath markup image are included below. (e) Kaplan Meier curve showing patient stratification based on median tumor stromal percentage. Representative images show the original images and markup for tumors with a high and low stromal percentage respectively. Green indicates regions classified as stroma, dark red indicates tumor epithelium, while yellow represents other classified tissue or whitespace.