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. 2017 Dec 4;7:16892. doi: 10.1038/s41598-017-16928-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of Ovalbumin-presenting recombinant MV_vac2. (a) Immunoblot analysis of Vero cells infected at an MOI of 0.03 with MV_vac2 or MV_vac2-gag expressing membrane-bound extracellular version of Ovalbumin (DisOva) compared to vaccine expressing no antigen (MV_vac2-ATU) in virus passages 3 (P3) or 10 (P10). Uninfected Vero cells served as control. Blots were probed as indicated. (b) Immunoelectron microscopic analysis of MLV-derived VLPs purified from supernatants of virus-infected Vero cells. VLPs displaying Ovalbumin or pure VLPs, as depicted above images, analyzed after fixation and probing for Ova. Arrows depict Ova-specific labelling. Scale bar: 100 nm. (c,d) Growth kinetics of recombinant MV (P3) on Vero cells infected at an MOI of 0.03 with indicated viruses. Cell-associated virus titers (c) as well as virus titers in the supernatant (d) of samples prepared at indicated time points post infection were titrated on Vero cells. Means and standard deviations of three independent experiments. dpi, days post infection; TCID₅₀, tissue culture infectious dose 50.