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. 2017 Dec 4;7:16885. doi: 10.1038/s41598-017-17173-9

Figure 3.

Figure 3

Quantitative RT-PCR analysis of ammonium nitrate-responsive genes. Total RNA extracted from roots of 17-day-old rice seedlings treated with ammonium nitrate for 0–24 h was used for qRT-PCR analysis to verify the expression of genes involved in nitrate transport (A), nitrate/nitrite assimilation (B), ferredoxin reduction (C), the pentose phosphate pathway (D), and amino acid transport (E). Relative expression indicates the fold-change of each gene as compared to that of control. Results are shown as means ± SD from three biological repeats.