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. 2017 Dec 4;7:16885. doi: 10.1038/s41598-017-17173-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regulation of nitrogen-responsive protein kinase/phosphatase genes by ammonium nitrate. Total RNA extracted from roots of 17-day-old rice seedlings treated with ammonium nitrate for 0–24 h was used for qRT-PCR to analyze the expression of protein kinase (A) and phosphatase (B) genes. Relative expression indicates the fold-change of each gene as compared to that of control. Results are shown as means ± SD from three biological repeats.