Table 1.

The quantity of opportunistic pathogens in sediments and water of storage tanks using qPCR in two replicates.

Matrix	Location ID	Acanthamoeba spp.	Vermamoeba vermiformis	Mycobacterium spp.	Pseudomonas aeruginosa	Legionella spp.	L. pneumophila (rtxA)	L. pneumophila (sidF)
Sediment (GN G−1: genome copy number per gram sediment)	D39S	5 ± 1	0	(2.73 ± 0.68) × 104	0	335 ± 31	0	0
	D40S	1 ± 1	4	(8.10 ± 1.16) × 103	0	923 ± 34	0	0
	D41S	28 ± 7	0	(8.00 ± 1.20) × 101	0	0	0	0
	D42S	0	0	(8.47 ± 10.04) × 103	0	0	0	0
	D43S	0	0	0	0	0	0	0
	D50S	0	14 ± 22	(2.00 ± 4.00) × 101	0	1 ± 3	0	0
	D53S	7	99 ± 60	(2.39 ± 2.24) × 103	0	282 ± 113	25 ± 51	85 ± 69
	D54S	3	120 ± 43	(2.57 ± 0.88) × 105	0	(7.20 ± 3.31) × 104	300 ± 38	173 ± 87
	Average	6	29	3.79 × 104	0	9.19 × 103	41	32
	Occurrence	63%	50%	88%	0	50%	25%	25%
Water (GN L−1: genome copy number per liter water)	D39w	0	0	(2.33 ± 3.28) × 105	70 ± 91	(1.24 ± 1.70) × 104	0	0
	D40w	0	0	(5.63 ± 7.74) × 104	0	(6.93 ± 9.76) × 104	0	0
	D41w	50	0	6.91 × 103	0	0	0	0
	D42w	0	0	3.37 × 104	0	0	0	0
	D43w	0	0	2.52 × 103	0	1.50 × 103	0	0
	D50w	0	480 ± 670	(5.07 ± 0.03)×103	2770 ± 14	(1.84 ± 1.46) × 104	82 ± 117	36 ± 52
	D53w	0	0	0	83 ± 117	0	0	0
	D54w	0	98 ± 136	(2.68 ± 2.84) × 103	371 ± 150	0	0	0
	Average	6	613	4.25 × 104	412	1.27 × 104	10	4
	Occurrence	13%	25%	88%	50%	50%	13%	13%