Table 1.
Sequences of the Phytochelatin-Related α-Peptides Used
| Designation | NCs solubility Water/Buffera | NCs pH stability 4.0/10.0b | Sequencesc |
|---|---|---|---|
| 1 | +/− | ND | Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 2 | +/− | ND | G-G-S-G-G-S-G-G-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 3 | −/− | −/− | G-R-R-G-E-G-R-R-G-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 4 | +/+ | +/+ | G-S-E-S-G-G-S-E-S-G-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 5 | +/+ | +/+ | Ac-G-S-E-S-G-G-S-E-S-G-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 6 | +/+ | +/+ | Suc-G-S-S-S-G-G-S-S-S-G-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 7 | +/+ | +/+ | K-G-S-E-S-G-G-S-E-S-G-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 8 | +/+ | +/+ | Biotin-G-S-E-S-G-G-S-E-S-G-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 9 | +/− | +/+ | PEG-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
| 10 | +/+ | −/+ | Ac-G-P-K-K-K-R-K-V-G-G-S-E-S-G-G-S-E-S-G-Cha-C-C-Cha-C-C-Cha-C-C-Cha-Cmd |
a
100 mM PBS buffer pH 7.2.
b
25 mM potassium hydrophthalate buffer pH 4.0 and 25 mM sodium bicarbonate buffer pH 10.0.
c
All sequences are written from N- to C-terminus.
Cmd: carboxamide; Ac: N-terminal acetylation; Suc: N-terminal succinylation; Cha: 3-cyclohexylalanine, PEG: hexaethyleneglycol. ND: not determined.