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. 2017 Nov 30;8:2032. doi: 10.3389/fpls.2017.02032

Table 1.

Photosynthetic parameters deduced by the JIP-test analysis of fluorescence transients.

0 DAY 5 DAY Definitions
SE S SC CK SE S SC CK
DATA EXTRACTED FROM THE RECORDED OJIP FLUORESCENCE TRANSIENT CURVES
F0 = F20μs 0.46a 0.47a 0.48a 0.49a 0.45a 0.46a 0.47a 0.49a Fluorescence at time t after onset of actinic illumination
FK 1.05a 1.08a 1.07a 0.98b 1.12ab 1.16a 1.13ab 1.05b Fluorescence value at 300 μs
FJ 1.25a 1.25a 1.27a 1.19a 1.27a 1.30a 1.31a 1.24a Fluorescence value at the J-step of OJIP
FI 1.70a 1.60b 1.73a 1.69a 1.65b 1.70ab 1.73a 1.70ab Fluorescence value at the I-step of OJIP
FP = FM 1.89a 1.86a 1.95a 1.89a 1.79b 1.87ab 1.92a 1.90a Fluorescence value at the peak of OJIP test
M0 1.61a 1.77a 1.61a 1.44b 2.01a 1.99a 1.82b 1.60c Approximate value of the initial slope of fluorescence transient curves
VJ 0.55ab 0.56a 0.54ab 0.51b 0.61a 0.60ab 0.58b 0.53c Relative variable fluorescence at J-step
Area 10.45a 15.41a 12.86a 10.45a 7.38b 11.82ab 14.43a 11.99ab The area above the chlorophyll fluorescence curve between Fo and Fm
N 21.96a 34.66a 26.11a 20.91a 17.99b 27.82ab 31.52a 25.38ab Number of QA redox turnovers until Fm is reached
SPECIFIC ENERGY FLUXES (PER ACTIVE PSII REACTION CENTER)
ABS/RC 0.65ab 0.75a 0.66ab 0.55b 0.93a 0.90a 0.79b 0.63c Absorbed photon flux per RC
TR0/RC 2.96b 3.16a 2.98b 2.84b 3.27ab 3.34a 3.16bc 3.00c Trapped excitation flux (leading to QA reduction) per RC
ET0/RC 1.35a 1.39a 1.37a 1.39a 1.27b 1.34ab 1.33ab 1.40a Electron transport flux (further than QA-) per RC
RE0/RC 0.08a 0.12a 0.09a 0.09a 0.05c 0.07bc 0.07ab 0.10a Electron flux reducing end electron acceptors at the PSI acceptor side, per RC
QUANTUM YIELDS AND EFFICIENCIES/PROBABILITIES
φP0 = TR0/ABS 0.74a 0.75a 0.75a 0.75a 0.75a 0.75a 0.75a 0.74a Maximum quantum yield for primary photochemistry
ψE0 = ET0/TR0 0.45ab 0.44b 0.46ab 0.49a 0.39c 0.40bc 0.42b 0.47a Efficiency/probability with which a PSII trapped electron is transferred from QA to QB
φE0 = ET0/ABS 0.34b 0.33b 0.35ab 0.37a 0.29c 0.30bc 0.32b 0.35a Quantum yield of the electron transport flux from QA to QB
σR0 = RE0/ET0 0.06b 0.08a 0.07ab 0.07ab 0.04c 0.05bc 0.05ab 0.07a Efficiency/probability with which an electron from QB is transferred until PSI acceptors
φR0 = RE0/ABS 0.10b 0.14a 0.11b 0.10b 0.07b 0.09ab 0.10ab 0.11a Quantum yield for reduction of end Electron acceptors at the PSI acceptor side
γRC 0.20ab 0.19b 0.20ab 0.21a 0.18b 0.19ab 0.19ab 0.20a Probability that a PSII Chl molecule functions as RC
RC/ABS 1.53b 1.35b 1.54b 1.82a 1.08c 1.11c 1.26b 1.58a Number of QA reducing RCs per PSII antenna Chl
PERFORMANCE INDEXES (PI, COMBINATION OF PARAMETERS)
PIABS 0.49b 0.38b 0.45b 0.61a 0.35b 0.38b 0.43ab 0.51a PI (potential) for energy conservation from exciton to the reduction of intersystem electron
PItotal 0.03a 0.04a 0.04a 0.04a 0.01b 0.02b 0.03b 0.04a PI (potential) for energy conservation from exciton to the reduction of PSI end acceptors

Each parameter is carefully calculated according to previous method (Yusuf et al., 2010). Subscript “0” denotes that the parameter refers to the onset of illumination. Values are given as the average of 3 replicates, and different letters denote statistic significant difference at P < 0.05 among the treatments by Tukey's multiple range tests. 0 and 5 day, respectively executed variance analysis. The underline values are all carefully selected to avoid Blindness on browsing miscellaneous data.