Table 1.
Photosynthetic parameters deduced by the JIP-test analysis of fluorescence transients.
0 DAY | 5 DAY | Definitions | |||||||
---|---|---|---|---|---|---|---|---|---|
SE | S | SC | CK | SE | S | SC | CK | ||
DATA EXTRACTED FROM THE RECORDED OJIP FLUORESCENCE TRANSIENT CURVES | |||||||||
F0 = F20μs | 0.46a | 0.47a | 0.48a | 0.49a | 0.45a | 0.46a | 0.47a | 0.49a | Fluorescence at time t after onset of actinic illumination |
FK | 1.05a | 1.08a | 1.07a | 0.98b | 1.12ab | 1.16a | 1.13ab | 1.05b | Fluorescence value at 300 μs |
FJ | 1.25a | 1.25a | 1.27a | 1.19a | 1.27a | 1.30a | 1.31a | 1.24a | Fluorescence value at the J-step of OJIP |
FI | 1.70a | 1.60b | 1.73a | 1.69a | 1.65b | 1.70ab | 1.73a | 1.70ab | Fluorescence value at the I-step of OJIP |
FP = FM | 1.89a | 1.86a | 1.95a | 1.89a | 1.79b | 1.87ab | 1.92a | 1.90a | Fluorescence value at the peak of OJIP test |
M0 | 1.61a | 1.77a | 1.61a | 1.44b | 2.01a | 1.99a | 1.82b | 1.60c | Approximate value of the initial slope of fluorescence transient curves |
VJ | 0.55ab | 0.56a | 0.54ab | 0.51b | 0.61a | 0.60ab | 0.58b | 0.53c | Relative variable fluorescence at J-step |
Area | 10.45a | 15.41a | 12.86a | 10.45a | 7.38b | 11.82ab | 14.43a | 11.99ab | The area above the chlorophyll fluorescence curve between Fo and Fm |
N | 21.96a | 34.66a | 26.11a | 20.91a | 17.99b | 27.82ab | 31.52a | 25.38ab | Number of QA redox turnovers until Fm is reached |
SPECIFIC ENERGY FLUXES (PER ACTIVE PSII REACTION CENTER) | |||||||||
ABS/RC | 0.65ab | 0.75a | 0.66ab | 0.55b | 0.93a | 0.90a | 0.79b | 0.63c | Absorbed photon flux per RC |
TR0/RC | 2.96b | 3.16a | 2.98b | 2.84b | 3.27ab | 3.34a | 3.16bc | 3.00c | Trapped excitation flux (leading to QA reduction) per RC |
ET0/RC | 1.35a | 1.39a | 1.37a | 1.39a | 1.27b | 1.34ab | 1.33ab | 1.40a | Electron transport flux (further than ) per RC |
RE0/RC | 0.08a | 0.12a | 0.09a | 0.09a | 0.05c | 0.07bc | 0.07ab | 0.10a | Electron flux reducing end electron acceptors at the PSI acceptor side, per RC |
QUANTUM YIELDS AND EFFICIENCIES/PROBABILITIES | |||||||||
φP0 = TR0/ABS | 0.74a | 0.75a | 0.75a | 0.75a | 0.75a | 0.75a | 0.75a | 0.74a | Maximum quantum yield for primary photochemistry |
ψE0 = ET0/TR0 | 0.45ab | 0.44b | 0.46ab | 0.49a | 0.39c | 0.40bc | 0.42b | 0.47a | Efficiency/probability with which a PSII trapped electron is transferred from QA to QB |
φE0 = ET0/ABS | 0.34b | 0.33b | 0.35ab | 0.37a | 0.29c | 0.30bc | 0.32b | 0.35a | Quantum yield of the electron transport flux from QA to QB |
σR0 = RE0/ET0 | 0.06b | 0.08a | 0.07ab | 0.07ab | 0.04c | 0.05bc | 0.05ab | 0.07a | Efficiency/probability with which an electron from QB is transferred until PSI acceptors |
φR0 = RE0/ABS | 0.10b | 0.14a | 0.11b | 0.10b | 0.07b | 0.09ab | 0.10ab | 0.11a | Quantum yield for reduction of end Electron acceptors at the PSI acceptor side |
γRC | 0.20ab | 0.19b | 0.20ab | 0.21a | 0.18b | 0.19ab | 0.19ab | 0.20a | Probability that a PSII Chl molecule functions as RC |
RC/ABS | 1.53b | 1.35b | 1.54b | 1.82a | 1.08c | 1.11c | 1.26b | 1.58a | Number of QA reducing RCs per PSII antenna Chl |
PERFORMANCE INDEXES (PI, COMBINATION OF PARAMETERS) | |||||||||
PIABS | 0.49b | 0.38b | 0.45b | 0.61a | 0.35b | 0.38b | 0.43ab | 0.51a | PI (potential) for energy conservation from exciton to the reduction of intersystem electron |
PItotal | 0.03a | 0.04a | 0.04a | 0.04a | 0.01b | 0.02b | 0.03b | 0.04a | PI (potential) for energy conservation from exciton to the reduction of PSI end acceptors |
Each parameter is carefully calculated according to previous method (Yusuf et al., 2010). Subscript “0” denotes that the parameter refers to the onset of illumination. Values are given as the average of 3 replicates, and different letters denote statistic significant difference at P < 0.05 among the treatments by Tukey's multiple range tests. 0 and 5 day, respectively executed variance analysis. The underline values are all carefully selected to avoid Blindness on browsing miscellaneous data.