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. 2017 Oct 21;108(12):2454–2461. doi: 10.1111/cas.13416

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Expression of aspartate‐β‐hydroxylase (ASPH) is upregulated when tamoxifen sensitivity is reduced. (a) Dose–response curves of 4‐hydroxytamoxifen (4‐OHT) in parental (●) and TamR (□) MCF7 cells. The x‐axis is expressed on a logarithmic scale. Mean ± SD of multiple samples (N = 6) is shown. (b) Quantitative RT‐PCR (qRT‐PCR) analysis of ASPH mRNA expression in parental and TamR MCF7 cells grown in maintenance medium. Mean ± SD of triplicate samples is shown. (c) Western blot analysis of ASPH and phosphorylated ERK1/2 and Akt in parental and TamR MCF7 cells grown in maintenance medium. (d) qRT‐PCR analysis of ASPH mRNA expression in MCF7 cells stimulated with maintenance medium for 12 h in the presence or absence of PD98059 or LY294002. Mean ± SD of triplicate samples is shown. **P < 0.01 (Student's t‐test).