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. 2017 Sep 26;108(12):2358–2365. doi: 10.1111/cas.13400

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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High levels of Wiskott‐Aldrich syndrome protein and SCAR Homolog (WASH) expression in primary esophageal squamous cell carcinoma (ESCC) predicts poor clinical outcome. (a) Relative mRNA levels of WASH expression in human ESCC tissues and adjacent normal tissues. (b) Comparison of WASH mRNA expression between ESCC patients at stage I/II and stage III/IV. (c) Immunohistochemistry (IHC) staining of ESCC specimens with antibody specific for WASH. Original magnification, ×200. (d) Kaplan–Meier analysis of overall survival was stratified by expression levels of WASH protein expression, with high (red) or low (black) WASH expression. (e) Comparison of WASH protein expression detected by IHC between patients at stage I/II or stage III/IV. (f) Comparison of WASH protein expression detected by IHC among patients with well to poor tumor cell differentiation. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.