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Spectral analyses of multiple RS/Aux II mutant of PqqE. (A) UV‐Vis spectra of the as‐purified and reconstituted C28S/C32S/C310S/C313S mutant and WT enzyme. (B, C) Mössbauer spectra of the as‐purified (B) and reconstituted (C) C28S/C32S/C310S/C313S mutant, recorded at 5 K. Simulated spectra are shown as follows: blue, [4Fe–4S]2+; green, [2Fe–2S]2+; red, free (noncluster) high‐spin Fe2+; black, the sum of the simulated spectra.
