Figure 1.

The effects of EGCG3″Me on the LPS‐induced osteoclast differentiation and bone resorption. (A) The chemical structure of EGCG3″Me. (B) Mouse POBs and BMCs were cocultured for 7 days with LPS (1 ng·mL⁻¹) and EGCG3″Me (3–30 μm). TRAP‐positive multinuclear osteoclasts were defined as osteoclasts. The upper panel shows the TRAP‐stained cells in each culture group. The data are expressed as the means ± SEM of three wells. (C) Mouse calvariae were cultured for 5 days with LPS (1 μg·mL⁻¹) and EGCG3″Me (3–30 μm). The bone‐resorbing activity was measured based on the calcium in the medium. The data are expressed as the means ± SEM of four cultures. A significant difference between the two groups was indicated; **P < 0.01 and ***P < 0.001 vs. control, ^## P < 0.01 and ^### P < 0.001 vs. LPS.