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. 2017 Nov 8;8:1507. doi: 10.3389/fimmu.2017.01507

Figure 5.

Figure 5

Overexpression of leptin inhibited autophagy in mice adipocytes. (A) Volcano plot of transcriptome in the white epididymal adipose tissue (eWAT) of mice injected with or without leptin (n = 3 each). Individual endoplasmic reticulum (ER) stress, autophagy, and inflammation signature genes were highlighted. Red: upregulated, blue: downregulated. (B) Gene expression level of leptin in adipocytes infected with pAd-Leptin or si-Leptin (n = 3). (C) Protein levels of phosphorylation of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), C/EBP homologous protein (Chop), glucose-regulated protein 78 (GRP78), and activating transcription factor 4 (Atf4) in adipocytes infected with pAd-Leptin or si-Leptin (n = 3). (D) Representative pictures of GFP-LC3 punctate structures in adipocytes expressing GFP-LC3, in the presence of pAd-Leptin or si-Leptin and pretreated with TM then incubated with or without bafilomycin A1 (BafA1). The nuclei were stained with DAPI shown in blue in adipocytes (n = 3). (E) Representative Western blots showing the protein levels of LC3II and SQSTM1 in adipocytes infected with pAd-Leptin or si-Leptin that pretreated with TM and followed treated with BafA1 or not (n = 3). Full scans of uncropped blots are included in Figure S3. Values are means ± SEM. *p < 0.05 compared with the control group, &p < 0.05 compared with the BafA1 group.