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. 2017 Nov 8;8:1507. doi: 10.3389/fimmu.2017.01507

Figure 6.

Figure 6

Leptin signal is essential for endoplasmic reticulum (ER) stress-induced autophagy and inflammation in mice adipocytes. Adipocytes were preinfected with pAd-leptin or si-leptin and incubated with tunicamycin (TM), then followed treated with 4-phenylbutyric acid (4-PBA, 5 mM for 2 h) or 3-methyladenine (3-MA, 50 mM for 1 h) (n = 3). (A) Representative pictures of autophagosome formation monitored by monodansylcadervarine (MDC) staining in adipocytes with or without 3-MA treatment. The nuclei were stained with DAPI shown in blue. (B) Representative Western blots showing the protein levels of LC3II, SQSTM1, and Atg5 in adipocytes treated with or without 3-MA. (C) Gene expressions of inflammation markers in adipocytes treated with or without 3-MA. (D) Representative Western blots showing the protein levels of LC3II, SQSTM1 and Atg5 in adipocytes treated with or without 4-PBA. (E) Gene expressions of inflammation markers in adipocytes treated with or without 4-PBA. Full scans of uncropped blots are included in Figure S3. Values are means ± SEM. *p < 0.05 compared with the control group, &p < 0.05 compared with the 3-MA group or 4-PBA group.